Prof. Dr. Jörg Andrä
Fakultät Life SciencesDepartment Biotechnologie
Professor für Organische Chemie und Biochemie
Lohbrügger Kirchstraße 65
21033 Hamburg
Raum S2.05
T +49.40.428 756317
joerg.andrae(@)haw-hamburg.de
Sprechzeiten: Dienstag 13-14 Uhr und nach Vereinbarung
Forschungsprojekte/-gebiete
DFG Einzelantrag
Significance of cellular surface factors for the selective cytotoxicity of antimicrobial peptides (AMPs) against cancer cells
The objectives of this project are to gain an understanding of the dynamics of the interactions of antimicrobial peptides with human cancer cells and the molecular basis for differential cytotoxicity and mode of action based on the hypothesis that the presence and/or shape of the cellular surface (glyco)-structures have a significant impact on peptide-cell interaction. We will comprehensively analyse peptide interactions with human cancer cell lines, which naturally or artificially differ in the surface exposition of negatively charged glycans (e.g. sialic acids) or in the thickness of the glycocalyx. The core instrumentation of this application is a metabolic chip biosensor, which enables the assessment of the dynamics of important cell physiological parameters. To correlate the biological activities of peptides with the presence and chemical structure of cellular surface molecules, the biological data will be complemented by peptide-membrane interaction studies, e.g. membrane binding, intercalation, and permeabilization, using artificial membranes composed of phospholipids and glycolipids to mimic the differently composed membranes of normal and aberrant cells.
Publikationen
1. Fukuoka, S., Richter, W., Howe, J., Andrä, J., Rössle, M., Alexander, C., Gutsmann, T., and Brandenburg, K. (2012) Innate Immunity 18, 302-317
2. Zweytick, D., Deutsch, G., Andrä, J., Blondelle, S. E., Vollmer, E., Jerala, R., and Lohner, K. (2011) J. Biol. Chem. 286, 21266-21276
3. Sanchez Gomez, S., Japelj, B., Jerala, R., Moriyon, I., Alonso, M. F., Leiva, J., Blondelle, S. E., Andrä, J., Brandenburg, K., Lohner, K., and Martinez de Tejada, G. (2011) Antimicrob. Agents Chemother. 55, 218-228
4. Drechlser, S., and Andrä, J. (2011) J. Bioenerget. Biomem. 43, 275-285
5. Andrä, J., Goldmann, T., Ernst, C. M., Peschel, A., and Gutsmann, T. (2011) J. Biol. Chem. 286, 18692-18700
6. Travkova, O. G., Andrä, J., Möhwald, H., and Brezesinski, G. (2010) ChemPhysChem 11, 3262-3268
7. Kowalski, I., Kaconis, Y., Andrä, J., Razquin-Olazarán, I., Martinez de Tejada, G., and Brandenburg, K. (2010) Prot. Pept. Lett. 17, 1328-1333
8. Hammer, M., Brauser, A., Olak, C., Brezesinski, G., Goldmann, T., Gutsmann, T., and Andrä, J. (2010) Biochem. J. 427, 477-488
9. Brandenburg, K., Howe, J., Sanchez Gomez, S., Garidel, P., Roessle, M., Andrä, J., Jerala, R., Zweytick, D., Lohner, K., Rappolt, M., Blondelle, S. E., Moriyon, I., and Martinez de Tejada, G. (2010) Antiinfec. Agents Med. Chem. 9, 9-22
10. Brandenburg, K., Garidel, P., Fukuoka, S., Howe, J., Koch, M. H., Gutsmann, T., and Andrä, J. (2010) Biophys. Chem. 150, 80-87
11. Hanulova, M., Andrä, J., Garidel, P., Olak, C., Howe, J., Funari, S. S., Gutsmann, T., and Brandenburg, K. (2009) Antinfect. Agents Med. Chem. 8, 17-27
12. Andrä, J., Hammer, M. U., Grötzinger, J., Jakovkin, I., Lindner, B., Vollmer, E., Fedders, H., Leippe, M., and Gutsmann, T. (2009) Biol. Chem. 390, 337-349
13. Sánchez-Gómez, S., Lamata, M., Leiva, J., Blondelle, S. E., Jerala, R., Andrä, J., Brandenburg, K., Moriyón, I., and Tejada, G. M. d. (2008) BMC Microbiol. 8:196
14. Olak, C., Muenter, A., Andrä, J., and Brezesinski, G. (2008) J. Pept. Sci. 14, 510-517
15. Gelhaus, C., Jacobs, T., Andrä, J., and Leippe, M. (2008) Antimicrob. Agents Chemother. 52, 1713-1720
16. Fukuoka, S., Howe, J., Andrä, J., Gutsmann, T., Rössle, M., and Brandenburg, K. (2008) Biochim. Biophys. Acta 1778, 2051-2057
17. Andrä, J., Jakovkin, I., Grötzinger, J., Hecht, O., Krasnosdembskaya, A. D., Goldmann, T., Gutsmann, T., and Leippe, M. (2008) Biochem. J. 410, 113-122
18. Andrä, J., Böhling, A., Gronewold, T. M. A., Schlecht, U., Perpeet, M., and Gutsmann, T. (2008) Langmuir 24, 9148-9153
19. Japelj, B., Zorko, M., Majerle, A., Pristovsek, P., Sanchez Gomez, S., Martinez de Tejada, G., Moriyon, I., Blondelle, S. E., Brandenburg, K., Andrä, J., Lohner, K., and Jerala, R. (2007) J. Am. Chem. Soc. 129, 1022-1023
20. Howe, J., Andrä, J., Moriyon, I., Garidel, P., Koch, M. H. J., Gutsmann, T., and Brandenburg, K. (2007) Biophys. J. 92, 2796-2805
21. Chen, X., Howe, J., Andrä, J., Rössle, M., Richter, W., Silva, A. P. G. d., Krensky, A. M., Clayberger, C., and Brandenburg, K. (2007) Biochim. Biophys. Acta 1768, 2421-2431
22. Andrä, J., Monreal, D., Martinez de Tejada, G., Olak, C., Brezesinski, G., Sanchez Gomez, S., Goldmann, T., Bartels, R., Brandenburg, K., and Moriyon, I. (2007) J. Biol. Chem. 282, 14719-14728
23. Andrä, J., Howe, J., Garidel, P., Rössle, M., Richter, W., Tejada, G. M. d., Moriyon, I., Bartels, R., Gutsmann, T., and Brandenburg, K. (2007) Biochem. J. 406, 297-307
24. Zweytick, D., Pabst, G., Abuja, P. M., Jilek, A., Blondelle, S. E., Andrä, J., Jerala, R., Monreal, D., Martinez de Tejada, G., and Lohner, K. (2006) Biochim. Biophys. Acta 1758, 1426-1435
25. Howe, J., Bauer, J., Andrä, J., Schromm, A. B., Ernst, M., Rössle, M., Zähringer, U., Rademann, J., and Brandenburg, K. (2006) FEBS J. 273, 5101-5112
26. Bruhn, H., Winkelmann, J., Andersen, C., Andrä, J., and Leippe, M. (2006) Dev. Comp. Immunol. 30, 597-606
27. Andrä, J., Rademann, J., Howe, J., Koch, M. H., Heine, H., Zähringer, U., and Brandenburg, K. (2006) Biol. Chem. 387, 301-310
28. Willumeit, R., Kumpugdee, M., Funari, S. S., Lohner, K., Pozo Navas, B., Brandenburg, K., Linser, S., and Andrä, J. (2005) Biochim. Biophys. Acta 1669, 125-134
29. Seydel, U., Schromm, A. B., Brade, L., Gronow, S., Andrä, J., Müller, M., Koch, M. H. J., Fukase, K., Kataoka, M., Hashimoto, M., Kusumoto, S., and Brandenburg, K. (2005) FEBS J. 272, 327-340
30. Schröder-Borm, H., Bakalova, R., and Andrä, J. (2005) FEBS Lett. 579, 6128-6134
31. Milkereit, G., Brandenburg, K., Gerber, S., Koch, M. H. J., Morr, M., Andrä, J., Seydel, U., and Vill, V. (2005) Chem. Phys. Lipids 135, 15-26
32. Jacobs, T., Andrä, J., Gaworski, I., Graefe, S., Mellenthin, K., Krömer, M., Halter, R., Borlak, J., and Clos, J. (2005) Med. Microbiol. Immunol. 194, 143-149
33. Garidel, P., Rappolt, M., Schromm, A. B., Howe, J., Lohner, K., Andrä, J., Koch, M. H. J., and Brandenburg, K. (2005) Biochim. Biophys. Acta 1715, 122-131
34. Brandenburg, K., Garidel, P., Schromm, A. B., Andrä, J., Kramer, A., Egmond, M., and Wiese, A. (2005) Eur. Biophys. J. 34, 28-41
35. Brandenburg, K., David, A., Howe, J., Koch, M. H. J., Andrä, J., and Garidel, P. (2005) Biophys. J. 88, 1845-1858
36. Andrä, J., Lohner, K., Blondelle, S. E., Jerala, R., Moriyon, I., Koch, M. H. J., Garidel, P., and Brandenburg, K. (2005) Biochem. J. 385, 135-143
37. Andrä, J., de Cock, H., Garidel, P., Howe, J., and Brandenburg, K. (2005) Med. Chem. 1, 537-546
38. Toman, R., Garidel, P., Andrä, J., Slaba, K., Hussein, A., Koch, M. H. J., and Brandenburg, K. (2004) BMC Biochem. 5:1
39. Kölln, J., Spillner, E., Andrä, J., Klensang, K., and Bredehorst, R. (2004) J. Immunol. 173, 5540-5545
40. Brandenburg, K., Hawkins, L., Garidel, P., Andrä, J., Müller, M., Heine, H., Koch, M. H. J., and Seydel, U. (2004) Biochemistry 43, 4039 - 4046
41. Andrä, J., Lamata, M., Martinez de Tejada, G., Bartels, R., Koch, M. H. J., and Brandenburg, K. (2004) Biochem. Pharmacol. 68, 1297-1307
42. Andrä, J., Koch, M. H. J., Bartels, R., and Brandenburg, K. (2004) Antimicrob. Agents Chemother. 48, 1593-1599
43. Andrä, J., Garidel, P., Majerle, A., Jerala, R., Ridge, R., Paus, E., Novitsky, T., Koch, M. H. J., and Brandenburg, K. (2004) Eur. J. Biochem. 271, 2037-2046
44. Andrä, J., Berninghausen, O., and Leippe, M. (2004) FEBS Lett. 564, 109-115
45. Schröder-Borm, H., Willumeit, R., Brandenburg, K., and Andrä, J. (2003) Biochim. Biophys. Acta 1612, 164-171
46. Brandenburg, K., Wagner, F., Müller, M., Heine, H., Andrä, J., Koch, M. H. J., Zähringer, U., and Seydel, U. (2003) Eur. J. Biochem. 270, 3171-3279
47. Brandenburg, K., Garidel, P., Andrä, J., Jürgens, G., Müller, M., Blume, A., Koch, M. H. J., and Levin, J. (2003) J. Biol. Chem. 278, 47660-47669
48. Andrä, J., Herbst, R., and Leippe, M. (2003) Dev. Comp. Immunol. 27, 291-304
49. Brandenburg, K., Jürgens, G., Andrä, J., Lindner, B., Koch, M. H., Blume, A., and Garidel, P. (2002) Eur. J. Biochem. 269, 5972-5981
50. Andrä, J., Halter, R., Kock, M. A., Niemann, H., Vogel, C. W., and Paul, D. (2002) Mol. Immunol. 39, 357-365
51. Andrä, J., Berninghausen, O., and Leippe, M. (2001) Med. Microbiol. Immunol. 189, 169-173
52. Andrä, J., and Leippe, M. (1999) Med. Microbiol. Immunol. 188, 117-124
53. Andrä, J., Berninghausen, O., and Leippe, M. (1997) Arch. Med. Res. 28, 156-157
54. Andrä, J., Berninghausen, O., Wülfken, J., and Leippe, M. (1996) FEBS Lett. 385, 96-100
55. Leippe, M., Andrä, J., Nickel, R., Tannich, E., and Müller-Eberhard, H. J. (1994) Mol. Microbiol. 14, 895-904
56. Leippe, M., Andrä, J., and Müller-Eberhard, H. J. (1994) Proc. Natl. Acad. Sci. USA 91, 2602-2606
57. Andrä, J., and Leippe, M. (1994) FEBS Lett. 354, 97-102
