Prof. Dr. Julien Béthune

Department of Biotechnology
Professor für Molekularbiologie und Zellkulturtechnik

Ulmenliet 20
21033 Hamburg

Room S2.24

T +49 40 428 75-6283



Bachelor Biotechnologie

  • Molekularbiologie + Praktikum
  • Mikrobiologie und Molekularbiologie Seminar

Master Pharmaceutical Biotechnology

  • Cell Culture Techniques + Practice
  • Host and Expression Systems

Focus areas/Expertise

  • Genome editing (CRISPR/Cas)
  • Gene silencing (RNAi)
  • Cellular assays
  • Proximity-dependent proteomics (BioID) 

Short bio

  • Diplôme d'Ingénieur Chimiste (Ecole Nationale Supérieure de Chimie de Montpellier) - 2000
  • Master of Science Biochemistry and Molecular Biology (University of Louisville, USA) - 2000
  • Promotion (Universität Heidelberg) - 2006
  • Wiss. Mitarbeiter (Universität Heidelberg) - 2006-2007
  • Laborleiter/Principal Scientist bei Novartis AG (Basel) - 2007-2008
  • Wissenschaftler am Friedrich Miescher Institute for Biomedical Research (Basel) - 2008-2013
  • Nachwuchsgruppenleiter (Universität Heidelberg) - 2013-2020
  • Professor für Zellkulturtechnik und Molekularbiologie an der HAW - seit 2020



Jain Goyal, M., Zhao, X., Bozhinova, M., Andrade-López, K., de Heus, C., Schulze-Dramac, S., Müller-McNicoll, M., Klumperman, J. and Béthune, J. (2020) A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells. Life Science Alliance, 3, e202000714.

Rafiee, M.R., Sigismondo, G., Kalxdorf, M., Forster, L., Brugger, B., Béthune, J. and Krijgsveld, J. (2020) Protease-resistant streptavidin for interaction proteomics. Molecular systems biology, 16, e9370.

Adolf, F., Rhiel, M., Hessling, B., Gao, Q., Hellwig, A., Béthune, J. and Wieland, F.T. (2019) Proteomic Profiling of Mammalian COPII and COPI Vesicles. Cell reports, 26, 250-265 e255.

Béthune, J., Jansen, R.P., Feldbrugge, M. and Zarnack, K. (2019) Membrane-Associated RNA-Binding Proteins Orchestrate Organelle-Coupled Translation. Trends in cell biology, 29, 178-188.

Egetemaier, S. and Béthune, J. (2019). Proteomik-Analyse von dynamischen Proteinkomplexen. Biospektrum (Springer Verlag) 01.19, 45-48

Schopp, I.M. and Béthune, J. (2018) Split-BioID - Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment. Journal of Visualized Experiments

Amaya Ramirez, C.C., Hubbe, P., Mandel, N. and Béthune, J. (2018) 4EHP-independent repression of endogenous mRNAs by the RNA-binding protein GIGYF2. Nucleic Acids Research, 46, 5792-5808.

Béthune, J. and Wieland, F.T. (2018) Assembly of COPI and COPII Vesicular Coat Proteins on Membranes. Annual Reviews of Biophysics, 47, 63-83.

Béthune, J. (2018). Analyzing context-specific protein complexes. G.I.T: Laboratory Journal (Wiley) 2/18, 18-19

Schopp, I.M., Amaya Ramirez, C.C., Debeljak, J., Kreibich, E., Skribbe, M., Wild, K. and Béthune, J. (2017) Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes. Nature Communications, 8, 15690.

Béthune, J., Artus-Revel, C.G. and Filipowicz, W. (2012) Kinetic analysis reveals successive steps leading to miRNA-mediated silencing in mammalian cells. EMBO Reports, 13, 716-723.

Beck, R., Sun, Z., Adolf, F., Rutz, C., Bassler, J., Wild, K., Sinning, I., Hurt, E., Brügger, B., Béthune, J. et al. (2008) Membrane curvature induced by Arf1-GTP is essential for vesicle formation. Proceedings of the National Academy of Sciences U S A, 105, 11731-11736.

Langer, J.D., Roth, C.M., Béthune, J., Stoops, E.H., Brügger, B., Herten, D.P. and Wieland, F.T. (2008) A conformational change in the alpha-subunit of coatomer induced by ligand binding to gamma-COP revealed by single-pair FRET. Traffic, 9, 597-607.

Sun, Z., Anderl, F., Frohlich, K., Zhao, L., Hanke, S., Brugger, B., Wieland, F. and Béthune, J. (2007) Multiple and stepwise interactions between coatomer and ADP-ribosylation factor-1 (Arf1)-GTP. Traffic, 8, 582-593.

Langer, J.D., Stoops, E.H., Béthune, J. and Wieland, F.T. (2007) Conformational changes of coat proteins during vesicle formation. FEBS Letters, 581, 2083-2088.

Béthune, J., Kol, M., Hoffmann, J., Reckmann, I., Brugger, B. and Wieland, F. (2006) Coatomer, the coat protein of COPI transport vesicles, discriminates endoplasmic reticulum residents from p24 proteins. Molecular and Cellular Biology, 26, 8011-8021.

Béthune, J., Wieland, F. and Moelleken, J. (2006) COPI-mediated transport. Journal of Membrane Biology, 211, 65-79.