Prof. Dr. Julien Béthune

Department Biotechnologie
Professor für Molekularbiologie und Zellkulturtechnik

Ulmenliet 20
21033 Hamburg

Raum S2.24

T +49 40 428 75-6283
E-Mail E-Mail



Bachelor Biotechnologie

  • Molekularbiologie + Praktikum
  • Mikrobiologie und Molekularbiologie Seminar

Master Pharmaceutical Biotechnology

  • Cell Culture Techniques + Lab
  • Host and Expression Systems


  • Genome editing (CRISPR/Cas)
  • Gene silencing (RNAi)
  • Cellular assays
  • Proximity-dependent proteomics (BioID) 


Chair of the examinations committees of the Master programs:

  • Biomedical Engineering
  • Pharmaceutical Biotechnology
  • Process Engineering
  • Renewable Energy Systems

Before you send me a request, please have a look at the FAQ site for examinations-related questions for these master programs:


Scientific reviewer:

- Deutsche Forschungsgemeinschaft (DFG, Germany)

- Agence Nationale de la Recherche (ANR, France)

- Biotechnology and Biological Sciences Research Council (BBSRC, UK)

- Fondation contre le cancer (Belgium Foundation against Cancer)

- Many scientific journals

Betreute Abschlussarbeiten/Doktorarbeiten

- many bachelor, master and PhD theses since 2013


  • Diplôme d'Ingénieur Chimiste (Ecole Nationale Supérieure de Chimie de Montpellier) - 2000
  • Master of Science Biochemistry and Molecular Biology (University of Louisville, USA) - 2000
  • PhD (Universität Heidelberg) - 2006
  • Postdoctoral scientist  at Universität Heidelberg - 2006-2007
  • Lab head/Principal Scientist bei Novartis AG (Basel) - 2007-2008
  • Postdoctoral scientist at the Friedrich Miescher Institute for Biomedical Research (Basel) - 2008-2013
  • Junior group leader (Universität Heidelberg) - 2013-2020
  • Professor for cell culture techniques and molecular biology at Hamburg University of Applied Sciences - since 2020


Kubitz, L., Bitsch, S., Zhao, X., Schmitt K., Deweid, L., Roehrig, A., Cappio Barazzone, E., Valerius, O., Kolmar, H. and Béthune, J. (2022) Engineering of ultraID, a compact and hyperactive enzyme for proximity-dependent biotinylation in living cells. Communications Biology 5, 657

Béthune J. (2021) Catalyzing the Understanding of Enzyme Kinetics. HINT–Heidelberg Inspirations for Innovative Teaching, 2: 7-28

Amaya Ramirez C., Egetemaier S. and Béthune J. (2021) Context-Specific and Proximity-Dependent Labeling for the Proteomic Analysis of Spatiotemporally Defined Protein Complexes with Split-BioID. Methods in Molecular Biology, 2247:303-318

Jain Goyal, M., Zhao, X., Bozhinova, M., Andrade-López, K., de Heus, C., Schulze-Dramac, S., Müller-McNicoll, M., Klumperman, J. and Béthune, J. (2020) A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells. Life Science Alliance, 3, e202000714.

Rafiee, M.R., Sigismondo, G., Kalxdorf, M., Forster, L., Brugger, B., Béthune, J. and Krijgsveld, J. (2020) Protease-resistant streptavidin for interaction proteomics. Molecular systems biology, 16, e9370.

Adolf, F., Rhiel, M., Hessling, B., Gao, Q., Hellwig, A., Béthune, J. and Wieland, F.T. (2019) Proteomic Profiling of Mammalian COPII and COPI Vesicles. Cell reports, 26, 250-265 e255.

Béthune, J., Jansen, R.P., Feldbrugge, M. and Zarnack, K. (2019) Membrane-Associated RNA-Binding Proteins Orchestrate Organelle-Coupled Translation. Trends in cell biology, 29, 178-188.

Egetemaier, S. and Béthune, J. (2019). Proteomik-Analyse von dynamischen Proteinkomplexen. Biospektrum (Springer Verlag) 01.19, 45-48

Schopp, I.M. and Béthune, J. (2018) Split-BioID - Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment. Journal of Visualized Experiments

Amaya Ramirez, C.C., Hubbe, P., Mandel, N. and Béthune, J. (2018) 4EHP-independent repression of endogenous mRNAs by the RNA-binding protein GIGYF2. Nucleic Acids Research, 46, 5792-5808.

Béthune, J. and Wieland, F.T. (2018) Assembly of COPI and COPII Vesicular Coat Proteins on Membranes. Annual Reviews of Biophysics, 47, 63-83.

Béthune, J. (2018). Analyzing context-specific protein complexes. G.I.T: Laboratory Journal (Wiley) 2/18, 18-19

Schopp, I.M., Amaya Ramirez, C.C., Debeljak, J., Kreibich, E., Skribbe, M., Wild, K. and Béthune, J. (2017) Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein complexes. Nature Communications, 8, 15690.

Béthune, J., Artus-Revel, C.G. and Filipowicz, W. (2012) Kinetic analysis reveals successive steps leading to miRNA-mediated silencing in mammalian cells. EMBO Reports, 13, 716-723.

Beck, R., Sun, Z., Adolf, F., Rutz, C., Bassler, J., Wild, K., Sinning, I., Hurt, E., Brügger, B., Béthune, J. et al. (2008) Membrane curvature induced by Arf1-GTP is essential for vesicle formation. Proceedings of the National Academy of Sciences U S A, 105, 11731-11736.

Langer, J.D., Roth, C.M., Béthune, J., Stoops, E.H., Brügger, B., Herten, D.P. and Wieland, F.T. (2008) A conformational change in the alpha-subunit of coatomer induced by ligand binding to gamma-COP revealed by single-pair FRET. Traffic, 9, 597-607.

Sun, Z., Anderl, F., Frohlich, K., Zhao, L., Hanke, S., Brugger, B., Wieland, F. and Béthune, J. (2007) Multiple and stepwise interactions between coatomer and ADP-ribosylation factor-1 (Arf1)-GTP. Traffic, 8, 582-593.

Langer, J.D., Stoops, E.H., Béthune, J. and Wieland, F.T. (2007) Conformational changes of coat proteins during vesicle formation. FEBS Letters, 581, 2083-2088.

Béthune, J., Kol, M., Hoffmann, J., Reckmann, I., Brugger, B. and Wieland, F. (2006) Coatomer, the coat protein of COPI transport vesicles, discriminates endoplasmic reticulum residents from p24 proteins. Molecular and Cellular Biology, 26, 8011-8021.

Béthune, J., Wieland, F. and Moelleken, J. (2006) COPI-mediated transport. Journal of Membrane Biology, 211, 65-79.